Identification of dual receptor-binding specific strains of human H5N1 viruses in China / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences
; (12): 104-108, 2012.
Artigo
em Inglês
| WPRIM (Pacífico Ocidental)
| ID: wpr-235564
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>Both the 2, 6 linkage and its topology on target cells are critical for the recognition by human influenza virus. The binding preference of avian flu virus H5N1 HA to the 2, 3-linked sialylated glycans is considered the major factor limiting its efficient infection and transmission in humans. To monitor potential adaptation of H5N1 virus in human population, the surveillance of receptor-binding specificity was undertaken in China.</p><p><b>METHODS</b>The binding specificity of 32 human H5N1 virus strains isolated from 2003 to 2009 was tested by 2, 3-specific sialidase-treated chicken red blood cell (CRBC) agglutination assay and a solid-phase direct binding assay with synthetic sialylglycopolymers.</p><p><b>RESULTS</b>Dual binding preference to 2, 3 and 2, 6-glycans were found in two strains A/Guangdong/1/06 (A/GD/1/06) and A/Guangxi/1/08 (A/GX/1/08). Though minor effect of short-2, 6-binding was detected in A/GX/1/08 at a low virus titer, both showed high affinity to the oligosaccharide at a high load. Notably both are of the long-2, 6-recognition, with the same topology as that of human H1N1 and H3N2 viruses.</p><p><b>CONCLUSION</b>The findings suggest that human H5N1 virus in China likely acquired the potential human-adaptation ability. Further research and surveillance on receptor-binding specificity of H5N1 viruses are required.</p>
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Polissacarídeos
/
Receptores Virais
/
Ácidos Siálicos
/
Testes de Hemaglutinação
/
Adaptação Biológica
/
Galinhas
/
China
/
Epidemiologia
/
Receptores de Superfície Celular
/
Influenza Humana
Tipo de estudo:
Estudo prognóstico
Limite:
Animais
/
Humanos
País/Região como assunto:
Ásia
Idioma:
Inglês
Revista:
Biomedical and Environmental Sciences
Ano de publicação:
2012
Tipo de documento:
Artigo