Anti-inflammatory mechanism of total glycosides of Acanthopanax Giraldii / 中国结合医学杂志
Chinese journal of integrative medicine
; (12): 210-215, 2009.
Article
em En
| WPRIM
| ID: wpr-236200
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the anti-inflammatory mechanisms of total glycosides of Acanthopanax Giraldii (TGA).</p><p><b>METHODS</b>The changes of prostaglandin E(2)(PGE(2)), tumor necrosis factor (TNF-alpha), nitric oxide (NO), and expressions of COX-1 mRNA and COX-2 mRNA in BALB/c mouse macrophages were observed by the radioimmunoassay, ELISA and nitric acid reduction and RT-PCR in the presence or absence of TGA.</p><p><b>RESULTS</b>(1) TGA could significantly decrease the production of PGE(2)and NO in mouse peritoneal macrophages. The inhibitory rate to LPS-induced PGE(2)production was 87% (TGA 100 mg/L, P<0.05, vs. LPS) and 62% (TGA 20 mg/L, P<0.05, vs. LPS), respectively. The inhibitory rate of NO production in mouse peritoneal macrophages was 49% (TGA 100 mg/L, P<0.05, vs. LPS) and 21% (TGA 20 mg/L, P<0.05 vs. LPS), respectively. TGA could not inhibit LPS-induced TNF-alpha production in mouse peritoneal macrophages. (2) TGA also inhibited the expression of COX-1 and COX-2 mRNA in RAW264.7 cells. The inhibitory rate of TGA to COX-1 mRNA was 22% (TGA 100 mg/L, P<0.05, vs. blank). The inhibitory rate of TGA to COX-2 mRNA was 55% (TGA 20 mg/L, P<0.05, vs. LPS) and 100% (TGA 100 mg/L, P<0.01 vs. LPS), respectively.</p><p><b>CONCLUSION</b>The anti-inflammatory mechanisms of TGA for inhibiting the production of NO and PGE(2)are through inhibiting COX-2 mRNA expression without TNF-alpha changes.</p>
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Base de dados:
WPRIM
Assunto principal:
Farmacologia
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Medicamentos de Ervas Chinesas
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Dinoprostona
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Regulação Enzimológica da Expressão Gênica
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Linhagem Celular
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Lipopolissacarídeos
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Fator de Necrose Tumoral alfa
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Macrófagos Peritoneais
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Eleutherococcus
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Biologia Celular
Limite:
Animals
Idioma:
En
Revista:
Chinese journal of integrative medicine
Ano de publicação:
2009
Tipo de documento:
Article