Screening and detecting of proteins interacting with mPem / 生物工程学报
Chinese Journal of Biotechnology
; (12): 125-130, 2006.
Article
em Zh
| WPRIM
| ID: wpr-237013
Biblioteca responsável:
WPRO
ABSTRACT
mPem, a homeobox gene, is expressed in a time and stage specific manner during murine ontogeny. Pem transcripts are abundant in 7- and 8-day mouse embryos, but decrease precipitously thereafter. On Day 9 they become abundant in placenta and yolk sac, persisting there until parturition. Although Pem transcripts are not detectable in most of adult tissues, they are present in reproductive system such as testis, epididymis and ovary. This indicates a important role for Pem during embryogenesis and reproductive development. To study the function of mPem protein, we used a GAL4 based yeast two-hybrid assay to screen a 7-day mouse embryo library with full-length of mPem. 3 proteins were found interacting with mPem protein. One of theses is Mdfic. We confirmed the interaction between mPem and Mdfic in yeast and in vitro. Mdfic, MyoD family inhibitor domain containing, encodes the myoD family inhibitor domain (I-mfa domain). The interaction between mPem and Mdfic suggested they maybe form the transcriptional regulator complex to regulate embryo differentiation.
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Ligação Proteica
/
Fatores de Transcrição
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Química
/
Genes Homeobox
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Proteínas de Homeodomínio
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Técnicas do Sistema de Duplo-Híbrido
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Desenvolvimento Embrionário
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Embrião de Mamíferos
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Genética
/
Metabolismo
Tipo de estudo:
Diagnostic_studies
/
Screening_studies
Limite:
Animals
/
Pregnancy
Idioma:
Zh
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2006
Tipo de documento:
Article