Rapid screening for MTHFR gene 677C>T polymorphism in Down syndrome using high resolution melting curve and pyrosequencing / 中华医学遗传学杂志
Chinese Journal of Medical Genetics
; (6): 528-533, 2013.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-237213
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To establish a rapid method for detecting MTHFR gene 677C>T polymorphisms with high-resolution melting curve method (HRM) and pyrosequencing.</p><p><b>METHODS</b>Peripheral blood samples were collected from 155 Down syndrome patients and 182 normal controls from Children's Hospital of Shanghai. The accuracy of three methods including regular HRM, internal control HRM and artificial heterozygosity HRM was compared. Meanwhile, allele frequencies in 10, 30 and 50 mixed samples were measured with pyrosequencing, and the results were compared with that of HRM.</p><p><b>RESULTS</b>Heterozygosity of 677C>T polymorphism could be distinguished by various HRM methods. However, homozygotes CC and TT were only identifiable by internal control HRM and artificial heterozygosity HRM. The accuracy of pyrosequencing for allele frequency has improved with increased sample number. When the number of mixed samples has exceeded 30, the difference between pyrosequencing results and actual values became less than 4%. TT genotype was more frequent in Down syndrome patients than controls (25.2% vs. 14.3%). No significant difference was found in T allele frequency between the two groups (44.9% vs. 40.1%).</p><p><b>CONCLUSION</b>Respectively, internal control HRM and pyrosequencing may be ideal methods for determination of genotypic and allelic frequencies.</p>
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Contexto em Saúde:
ODS3 - Saúde e Bem-Estar
/
ODS3 - Meta 3.4 Reduzir as mortes prematuras devido doenças não transmissíveis
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ODS3 - Meta 3.2 Reduzir as mortes de recém nascidos e crianças com menos de 5 anos
Problema de saúde:
Meta 3.2: Reduzir as mortes de recém nascidos e crianças com menos de 5 anos
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Anomalias Congênitas e Cromossômicas
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Cuidados de Saúde Neonatal
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Análise de Sequência de DNA
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Mutação Puntual
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Síndrome de Down
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Polimorfismo de Nucleotídeo Único
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Metilenotetra-Hidrofolato Redutase (NADPH2)
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Temperatura de Transição
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Diagnóstico
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Genética
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Métodos
Tipo de estudo:
Estudo diagnóstico
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Estudo de rastreamento
Limite:
Criança
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Criança, pré-escolar
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Feminino
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Humanos
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Masculino
Idioma:
Chinês
Revista:
Chinese Journal of Medical Genetics
Ano de publicação:
2013
Tipo de documento:
Artigo