Lentiviral vector-mediated short hairpin RNA targeting survivin inhibits abdominal growth of human endometrium xenograft in nude mice / 南方医科大学学报
Journal of Southern Medical University
; (12): 281-283, 2015.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-239195
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJEVTIVE</b>To investigate the inhibitory effect of lentiviral vector-mediated short hairpin RNA targeting survivin (LV-survivin shRNA) on the growth of human endometrium xenograft in the abdominal cavity of nude mice.</p><p><b>METHODS</b>The endometrium xenografts from 8 women with endometriosis were injected into the peritoneal cavities of 45 nude mice. The mice were then randomly assigned to receive intraperitoneal injection of LV-survivin shRNA, pGCL-NC-GFP (negative control) or PBS (blank control). Two weeks later, the number and morphometry of endometriotic lesions were quantified and the expression of survivin protein were detected by immunohistochemistry.</p><p><b>RESULTS</b>The formation of endometriotic lesions was significantly suppressed in mice receiving LV-survivin shRNA injection as compared with those in the two control groups (P/0.001). The mice in LV-survivin-shRNA group showed significantly down-regulated expression levels of survivin protein compared with those in the negative and blank control groups, presenting also necrosis in the endometriosis-like lesions in microscopic observation.</p><p><b>CONCLUSION</b>Lentiviral vector-mediated shRNA can effectively inhibit the expression of survivin in human endometrium xengrafts and suppress the formation and growth of endometriotic lesions in the abdominal cavities of nude mice.</p>
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Transplante
/
RNA Interferente Pequeno
/
Endometriose
/
Endométrio
/
Proteínas Inibidoras de Apoptose
/
Xenoenxertos
/
Vetores Genéticos
/
Genética
/
Camundongos Nus
/
Proteínas Associadas aos Microtúbulos
Limite:
Animais
/
Feminino
/
Humanos
Idioma:
Chinês
Revista:
Journal of Southern Medical University
Ano de publicação:
2015
Tipo de documento:
Artigo