Overexpression of PPARγ induces adipogenic steatosis in mouse primary hepatocytes / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
; (6): 68-74, 2016.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-239620
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of PPARγ overexpression on steatosis in mouse primary hepatocytes.</p><p><b>METHODS</b>Primary hepatocytes isolated from C57BL/6J mice were infected with either Ad/LacZ or Ad/PPARγ for 48 h. Steatosis of the primary hepatocytes was checked by Oil Red O staining. The mRNA and protein expression of adipocyte-specific genes PPARγ, aP2 and CideA were analyzed by using RT Real-time PCR and Western Blot.</p><p><b>RESULTS</b>Primary hepatocytes were small and even. Hepatocyte nuclei were round with dispersed chromatin and prominent nucleoli. Accumulated lipid droplets were observed in Ad/PPARγ-infected hepatocytes, but in Ad/LacZ-infected hepatocytes. Moreover, compared with Ad/LacZ-infected hepatocytes, the mRNA expression of PPARγ, aP2, FGF21 and CideA in Ad/PPARγ-infected hepatocytes were significantly induced, the protein expression of PPARγ and its target aP2 strongly increased.</p><p><b>CONCLUSION</b>over expression of PPARγ induces adipogenic steatosis in mouse primary hepatocytes.</p>
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Patologia
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Transfecção
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Células Cultivadas
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Adipócitos
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Hepatócitos
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PPAR gama
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Adipogenia
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Fígado Gorduroso
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Vetores Genéticos
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Metabolismo
Limite:
Animais
Idioma:
Chinês
Revista:
Journal of Zhejiang University. Medical sciences
Ano de publicação:
2016
Tipo de documento:
Artigo