Effect of shenmai injection on L-type calcium channel of diaphragmatic muscle cells in rats / 中国中西医结合杂志
Chinese Journal of Integrated Traditional and Western Medicine
; (12): 599-602, 2003.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-240911
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To explore the effect of Shenmai Injection (SMI) on L-type calcium channel of diaphragmatic muscle cells in rats.</p><p><b>METHODS</b>Single diaphragmatic muscle cell of rats was obtained by the acute enzyme isolation method and the standard whole-cell patch clamp technique was used to record the inward peak L-type calcium current (IPLC) and current-voltage relationship curve of diaphragmatic muscle cells of 7 rats, and to compare the effects of SMI in various concentrations on them.</p><p><b>RESULTS</b>When keeping the electric potential at -80 mV, stimulation frequency 0.5 Hz, clamp time 300 ms, stepped voltage 10 mV, and depolarized to +60 mV, 10 microliters/ml of SMI could only cause the mean IPLC of rat's diaphragmatic muscle cells increased from -6.9 +/- 0.6 pA/pF to -7.5 +/- 0.7 pA/pF, the amplification being (9.2 +/- 2.8)%, comparison between those of pre-treatment and post-treatment showed insignificant difference. But when the concentration of SMI increased to 50 microliters/ml and 100 microliters/ml, the mean IPLC increased to -8.4 +/- 0.6 pA/pF and -9.2 +/- 0.6 pA/pF, respectively, and the amplification was (22.4 +/- 1.7)% and (34.6 +/- 4.6)% respectively, showing significant difference to that of pre-treatment (P < 0.05). However, SMI showed no significant effect on maximal activation potential and reversal potential.</p><p><b>CONCLUSION</b>SMI can activate the calcium channel of diaphragmatic muscle cells in rats, increase the influx of Ca2+, so as to strengthen the contraction of diaphragmatic muscle, which may be one of the ionic channel mechanisms of SMI in treating diaphragmatic muscle fatigue in clinical practice.</p>
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Diafragma
/
Medicamentos de Ervas Chinesas
/
Extratos Vegetais
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Ratos Wistar
/
Técnicas de Patch-Clamp
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Fibras Musculares Esqueléticas
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Canais de Cálcio Tipo L
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Combinação de Medicamentos
/
Metabolismo
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Integrated Traditional and Western Medicine
Ano de publicação:
2003
Tipo de documento:
Artigo