Comparison between the post-column derivatization with bromine by HPLC and the fluorometric analysis for determination of aflatoxins in medicinal herbs and plant extracts / 药学学报
Acta Pharmaceutica Sinica
; (12): 997-1000, 2004.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-241374
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>AIM</b>To compare the post-column derivatization technique (IAC-PCD-HPLC) for the determination of aflatoxins B1, B2, G1 and G2 and the rapid procedure with fluorometric analysis (SFB) for the determination of total aflatoxins.</p><p><b>METHODS</b>The method of post-column derivatization with bromine by HPLC consisted of extraction of the sample with MeOH-H2O (7030) followed by clean-up of the extracts with immunoaffinity columns and finally, HPLC determination with fluorescence detection. Aflatoxins B1 and G1 were determined as their bromine derivatives, produced in an on-line post-column derivatization system. In SFB method, samples were ground and extracted with methanol-water (7030). A portion of the extract was cleaned up by passage through a immunoaffinity column, One mL of purified extract was derivatized with a bromine reagent, and fluorescence of the solution was immediately quantified with a calibrated fluorometer containing a broad wavelength pulsed xenon light source.</p><p><b>RESULTS</b>In IAC-HPLC method, the overall average recoveries for three different medicinal herbs spiked at levels of 1.3 and 2.6 ng x g(-1) of total aflatoxins ranged from 93% to 97%. The detection limit was 0. 06 microg x kg(-1) for both G2 and B2 and 0.20 microg x kg(-1) for both G1 and B1, based on a signal/noise 31 and the precision (within-laboratory relative standard deviation) ranged from 0.8% to 1.4%. Each of aflatoxins B1, B2, G1 and G2 in 39 kind medicinal materials were determined by IAC-PCD-HPLC, and the total aflatoxins were determined by SFB.</p><p><b>CONCLUSION</b>The SFB method is not the suitable method for the determination of total aflatoxins in medicinal herbs and plant extracts.</p>
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Plantas Medicinais
/
Espectrometria de Fluorescência
/
Bromo
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Medicamentos de Ervas Chinesas
/
Química
/
Cromatografia Líquida de Alta Pressão
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Aflatoxina B1
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Aflatoxinas
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Codonopsis
/
Prunus
Idioma:
Chinês
Revista:
Acta Pharmaceutica Sinica
Ano de publicação:
2004
Tipo de documento:
Artigo