The role of IL-22 on thymus recovery and its function in mice following allogeneic bone marrow transplantation / 中华血液学杂志
Chinese Journal of Hematology
; (12): 822-825, 2014.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-242051
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To explore the role of IL-22 on the recovery and function of thymus from graft-versus host disease (GVHD) mice after allogeneic bone marrow transplantation (allo-BMT).</p><p><b>METHODS</b>GVHD model was established by using of recipient male BALB/c and donor male C57BL/6 mice(6-8 W) respectively. The mice were divided into normal group, GVHD with IL-22 group (BS+IL-22) and without IL-22 group (BS+PBS). Numbers of thymus cells were detected at different time points. The ratio of T cell subsets from thymus was observed by flow cytometry. Percentages of IFN-γ-producing and IL-17-producing CD4+ T or CD8+ T cells were detected.</p><p><b>RESULTS</b>The total number of thymus cells in BS+IL-22 mice [(14.6±5.1)×10⁴] was significantly higher than that in BS+PBS mice [(6.2±2.9)×10⁴] at 14 days after allo-BMT. Thymus cells in BS+IL-22 mice expanded continuously and reached at the level of normal mice, which were still higher than that in BS+PBS group. Although there was no impact on the ratio of mature CD4+ and CD8+ T cell from thymus, the percentage of immature CD4+CD8+ T cell increased obviously in mice treated with IL-22. Percentages of IFN-γ+CD4+ T cell [Th1(2.42±0.75)%] and IFN-γ+CD8+ T cell [Tc1(5.44±0.47)%] were up-regulated by IL-22 treatment, whereas no changes were detected in IL-17+CD4+ T cell (Th17) and IL-17+CD8+ T cell (Tc17).</p><p><b>CONCLUSION</b>IL-22 accelerates the progress of thymus recovery, and increases the IFN-γ-producing ability of thymus CD4+ and CD8+ T cells from GVHD mice.</p>
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Timo
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Transplante Homólogo
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Linfócitos T CD4-Positivos
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Subpopulações de Linfócitos T
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Transplante de Medula Óssea
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Interleucinas
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Linfócitos T CD8-Positivos
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Interleucina-17
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Alergia e Imunologia
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Doença Enxerto-Hospedeiro
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Hematology
Ano de publicação:
2014
Tipo de documento:
Artigo