Expression of human G6PD gene in K562 cells mediated by retroviral vector / 中华实验和临床病毒学杂志

ABSTRACT

<p><b>OBJECTIVE</b>This study aimed to investigate the feasibility of gene therapy for severe G6PD deficiency.</p><p><b>METHODS</b>The recombinant retroviral vector bearing normal human G6PD cDNA was constructed and transferred into the erythroleukemia cell line K562. Author identified the integration of NeoR gene in the targeted cellular DNA by means of specific PCR. Quantitative method was used to measure the expression of G6PD in the targeted cells.</p><p><b>RESULTS</b>Construction of the recombinant retroviral vector was successfully established. PCR indicated the integration of NeoR gene in the targeted genomic DNA of the cells. The vector was also shown to be capable of expressing the foreign gene compared to the control (P<0.01).</p><p><b>CONCLUSIONS</b>The recombinant retroviral vector is competent for transferring and expressing the G6PD gene.</p>