Study of genes involved in chronic myeloid leukemia with t (3; 21) (q26; q22) in blastic crisis / 中华血液学杂志
Chinese Journal of Hematology
; (12): 310-313, 2006.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-243955
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To explore genes involved in chronic myeloid leukemia (CML) with t (3; 21) (q26; q22) chromosome translocation in blastic crisis.</p><p><b>METHODS</b>A case of CML patient with t (3; 21) (q26; q22) in blastic crisis was reported. AML1 and bcr/abl genes were detected by FISH in interphase and metaphase cells. Genes involved in t (3; 21) (q26; q22) were analysed by RT-PCR and sequencing.</p><p><b>RESULTS</b>AML1 gene hybridization signal was detected in der (3) and der (21) chromosomes. AML1-Evi1, AML1-MDS1-Evi1, AML1-EAP fusion transcripts and Evi1 gene were detected in mRNA level, but no AML1-Evi1 fusion transcript. The mRNA expression level of AML1-MDS1-Evi1 fusion gene was 1.58 and 1.54 times higher than that of AML1-MDS1 and AML1-EAP, respectively. The mRNA expression level of Evi1 gene of the patient was 2.71 times higher than that of HEL cell line.</p><p><b>CONCLUSION</b>t (3; 21) (q26; q22) resulted in the AML1-MDS1-Evi1, AML1-MDS1, AML1-EAP fusion transcripts, and Evi1 gene was also activated by the translocation. These secondary aberrations should be the molecular basis of CML patient with t (3; 21) (q26; q22) in blastic crisis.</p>
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Patologia
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Fatores de Transcrição
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Translocação Genética
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Cromossomos Humanos Par 3
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Cromossomos Humanos Par 21
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Proto-Oncogenes
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Leucemia Mielogênica Crônica BCR-ABL Positiva
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Crise Blástica
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Proteínas de Fusão Oncogênica
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Proteínas de Fusão bcr-abl
Limite:
Adulto
/
Humanos
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Masculino
Idioma:
Chinês
Revista:
Chinese Journal of Hematology
Ano de publicação:
2006
Tipo de documento:
Artigo