Investigation on long-term survival of transplanted bone marrow mesenchymal stem cells in infarcted myocardium of rats / 中华心血管病杂志
Chinese Journal of Cardiology
; (12): 171-175, 2011.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-244029
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the quantification and distribution of survival transplanted bone marrow mesenchymal stem cells (BMSCs) in infarcted myocardium of rats.</p><p><b>METHODS</b>BMSCs of male SD rats were isolated, purified and proliferated. Female SD rats were subjected to occlusion of left anterior descending artery, 1.2 × 10(6) BMSCs were injected into the centre zone of the infarct region 1 h after left anterior descending artery occlusion. Myocardial tissue was harvested randomly at 1, 3 and 6 (n = 18) weeks after transplantation. Transplanted cells were labeled with Hoechst33342 (n = 5) and Brdu (n = 5). Sry sequence of Y chromatosome in male rat was analyzed by real-time PCR in the tissue (n = 8).</p><p><b>RESULTS</b>FACS analysis showed that BMSCs expressed homogenous with CD44+/CD34-. Hoechst33342 or Brdu labeled positive cells were found in localized accumulation and some of them migrated to the margin of the infarct region, and vessel-like structure could be observed in the host hearts. The real-time PCR demonstrated that the survival rates of engrafted BMSCs were 7.88%, 7.82% and 8.73% respectively at 1, 3 and 6 weeks after transplantation.</p><p><b>CONCLUSION</b>BMSCs could survive in the host infarcted myocardium for more than one month and the survival rate was around 8% from the first week to the sixth week after transplantation.</p>
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Cirurgia Geral
/
Transplante de Medula Óssea
/
Ratos Sprague-Dawley
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Biologia Celular
/
Transplante de Células-Tronco Mesenquimais
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Células-Tronco Mesenquimais
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Sobrevivência de Enxerto
/
Infarto do Miocárdio
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Cardiology
Ano de publicação:
2011
Tipo de documento:
Artigo