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Expression and clinical significance of nerve growth factor in primary liver cancer / 中华肝脏病杂志
Chinese Journal of Hepatology ; (12): 121-124, 2013.
Artigo em Chinês | WPRIM (Pacífico Ocidental) | ID: wpr-246735
Biblioteca responsável: WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To determine the expression of nerve growth factor (NGF) in hepatic tissues and serum of patients with primary liver cancer (PLC), and to investigate the relationship of serum NGF levels with clinicopathological features of PLC.</p><p><b>METHODS</b>Hepatocellular carcinoma (HCC) samples and patient-matched tumor-adjacent liver samples were collected from 26 PLC patients to assess the mRNA and protein expressions of NGF by reverse transcription-PCR, western blotting (b-actin normalized), and immunohistochemistry. In addition, serum samples were collected from 40 PLC patients, 40 liver cirrhosis patients, 40 chronic hepatitis patients (including hepatitis B or C virus infections), and 30 healthy (normal) controls. The serum levels of NGF were measured by enzyme-linked immunosorbent assay. Intergroup differences were assessed by the t-test, and correlation with sex, age, presence of cirrhosis, tumor size, and TNM classification were assessed by the Kruskal-Wallis H test followed the Mann-Whitney U test.</p><p><b>RESULTS</b>HCC tissues showed higher mRNA and protein expressions of NGF than the corresponding tumor-adjacent non-HCC tissues. Hepatic NGF expression was mainly localized to the tumor cell cytoplasm. Serum NGF expression was significantly higher in PLC patients (33.86+/-16.11 pg/ml) and cirrhosis patients (20.57+/-9.73 pg/ml) than in normal controls (11.13+/-6.12 pg/ml) and chronic hepatitis patients (13.20+/-6.23 pg/ml) (P less than 0.01). Furthermore, when the PLC patients were stratified according to tumor size and TNM stage, the serum NGF level was found to be significantly higher in patients with tumors more than 5 cm (vs. less than 5 cm; U=83.000, P=0.002) or of TNM stage III/IV (vs. stage I/II; U=103.500, P=0.009).</p><p><b>CONCLUSION</b>Elevated expression of NGF in liver cancer tissues and serum of PLC patients is related with tumor size and TNM staging. These findings suggest that NGF may play a role in HCC tumorigenesis and/or that serum NGF may represent a prognostic marker of PLC.</p>
Assuntos
Texto completo: Disponível Base de dados: WPRIM (Pacífico Ocidental) Assunto principal: Patologia / Prognóstico / Sangue / Imuno-Histoquímica / Estudos de Casos e Controles / Carcinoma Hepatocelular / Fator de Crescimento Neural / Neoplasias Hepáticas / Metabolismo Tipo de estudo: Estudo observacional / Estudo prognóstico Limite: Adolescente / Adulto / Idoso / Feminino / Humanos / Masculino Idioma: Chinês Revista: Chinese Journal of Hepatology Ano de publicação: 2013 Tipo de documento: Artigo
Texto completo: Disponível Base de dados: WPRIM (Pacífico Ocidental) Assunto principal: Patologia / Prognóstico / Sangue / Imuno-Histoquímica / Estudos de Casos e Controles / Carcinoma Hepatocelular / Fator de Crescimento Neural / Neoplasias Hepáticas / Metabolismo Tipo de estudo: Estudo observacional / Estudo prognóstico Limite: Adolescente / Adulto / Idoso / Feminino / Humanos / Masculino Idioma: Chinês Revista: Chinese Journal of Hepatology Ano de publicação: 2013 Tipo de documento: Artigo
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