Establishment of NCAM L1 minigene model and its splicing patterns in different cell lines / 浙江大学学报·医学版

ABSTRACT

<p><b>OBJECTIVE</b>To establish a minigene model of neural cell adhesion molecule L1 (NCAM L1) gene and to study its splicing patterns in different cell lines.</p><p><b>METHODS</b>Using human genetic cDNA as template, the NCAM L1 minigene fragment was amplified and inserted into eukaryotic expression vector. The minigene was transfected into 4 cell lines and the splicing patterns of NCAM L1 minigene in these cell lines were studied.</p><p><b>RESULTS</b>The splicing patterns of NCAM L1 minigene were different in individual cell lines. In PFSK and Hela cell lines, two splicied isoforms were generated but in COS-1 and R28 cell lines, only one isoform existed.</p><p><b>CONCLUSION</b>NCAM L1 minigene model can be used in alternative splicing analysis.</p>