Phenylhexyl isothiocyanate (PHI) regulates histone methylation and acetylation and induces apoptosis in SMMC-7721 cells / 中华肝脏病杂志

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the effects of PHI on histone acetylation and methylation in hepatocellular carcinoma line SMMC-7721 cells.</p><p><b>METHODS</b>Apoptosis was measured by TUNNEL assay. Histone methylation and acetylation were detected by Western blot.</p><p><b>RESULTS</b>PHI inhibited cells growth and induced apoptosis. PHI treatment resulted in increased acetylation of histone H3 and H4 , elevated level of histone H3 lysine 4 methylation, and decreased level of histone H3 lysine 9 methylation.</p><p><b>CONCLUSIONS</b>PHI can modulate both histone acetylation and methylation, which could remodel chromatin structure. PHI may be a novel anticancer drug.</p>