Effect of quercetin on glioma cell U87 apoptosis and feedback regulation of MDM2-p53 / 南方医科大学学报
Journal of Southern Medical University
; (12): 686-689, 2014.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-249380
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of quercetin on apoptosis and feedback regulation of MDM2-p53 in multiform glioblastoma U87 cells in vitro.</p><p><b>METHODS</b>U87 cells exposed to different concentrations of quercetin (50, 100, and 150 µmol/L) were examined with flow cytometry, RT-PCR and Western blotting for detecting the cell apoptosis, MDM2 mRNA expression, and p53 and caspase-3 expressions.</p><p><b>RESULTS</b>Quercetin induced obvious apoptosis in U87 cells in a concentration-dependent manner, with apoptosis rates of (12.40∓0.70)% at Q0, (22.53∓0.72)% at Q50, (29.06∓0.81)% at Q100, and (31.5∓0.45)% at Q150. Quercetin significantly increased the expressions of MDM2 mRNA and active caspase-3 protein but decreased the expression of p53 in the cells.</p><p><b>CONCLUSION</b>Quercetin promotes the apoptosis of multiform glioblastoma U87 cells mediated by caspase-3 and influences the feedback balance of MDM2-p53.</p>
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Patologia
/
Farmacologia
/
Quercetina
/
Proteína Supressora de Tumor p53
/
Apoptose
/
Linhagem Celular Tumoral
/
Proteínas Proto-Oncogênicas c-mdm2
/
Caspase 3
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Glioma
/
Metabolismo
Limite:
Humanos
Idioma:
Chinês
Revista:
Journal of Southern Medical University
Ano de publicação:
2014
Tipo de documento:
Artigo