Preimplantation genetic diagnosis of chromosome translocations by analysis of polymorphic short tandem repeats
Singapore medical journal
; : 648-654, 2012.
Artigo
em Inglês
| WPRIM (Pacífico Ocidental)
| ID: wpr-249646
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>INTRODUCTION</b>We aimed to develop and implement a short tandem repeat (STR) polymerase chain reaction alternative to fluorescence in situ hybridisation (FISH) for the preimplantation genetic diagnosis (PGD) of chromosomal translocations.</p><p><b>METHODS</b>Selected informative STRs located on translocated arms of relevant chromosomes were used to discriminate between normal and unbalanced chromosome states in each embryo.</p><p><b>RESULTS</b>PGD cycles were performed on five couples where one spouse carried a balanced translocation. 27 embryos were analysed, of which 12 were normal/balanced, 12 were abnormal/unbalanced and three were indeterminate. Four PGD cycles proceeded to embryo transfer, of which two led to pregnancy. The first pregnancy showed a normal male karyotype, and a healthy baby was delivered at term. A second pregnancy unexpectedly miscarried in the second trimester from unknown causes.</p><p><b>CONCLUSION</b>STR analysis is a simple and suitable alternative to FISH for detecting unbalanced chromosomal states in preimplantation embryos.</p>
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Polimorfismo Genético
/
Translocação Genética
/
Resultado da Gravidez
/
Fertilização in vitro
/
Reação em Cadeia da Polimerase
/
Repetições de Microssatélites
/
Diagnóstico Pré-Implantação
/
Genética
/
Métodos
Tipo de estudo:
Estudo diagnóstico
Limite:
Feminino
/
Humanos
/
Masculino
/
Gravidez
Idioma:
Inglês
Revista:
Singapore medical journal
Ano de publicação:
2012
Tipo de documento:
Artigo