Investigation of the degradation and the mechanisms of artificial basement membrane co-cultured with oral carcinoma-associated fibroblasts / 华西口腔医学杂志
West China Journal of Stomatology
; (6): 223-225, 2007.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-249738
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the degradation of artificial basement membrane (matrigel) co-cultured with oral carcinoma-associated fibroblasts (CAFs) and its possible mechanism.</p><p><b>METHODS</b>CAFs and normal fibroblasts (NFs) were incubated on matrigel for 24, 48, 72 h. Equivalent amounts of conditioned medium were collected and assayed for total protein, hydroxyproline and matrix metalloproteinase-2 (MMP-2), matrix metalloproteinase-9 (MMP-9) activity by gelatin zymography.</p><p><b>RESULTS</b>Oral CAFs were superior to oral NFs in total protein and hydroxyproline density, CAFs present more pro-MMP-2 and activated MMP-2.</p><p><b>CONCLUSION</b>CAFs were superior to NFs in degradation of matrigel. CAFs might play a key role in the reconstitution of extracellular matrix and the progression of tumor.</p>
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Membrana Basal
/
Neoplasias Bucais
/
Gelatinases
/
Técnicas de Cocultura
/
Metaloproteinase 2 da Matriz
/
Metaloproteinase 9 da Matriz
/
Precursores Enzimáticos
/
Fibroblastos
/
Membranas Artificiais
Limite:
Humanos
Idioma:
Chinês
Revista:
West China Journal of Stomatology
Ano de publicação:
2007
Tipo de documento:
Artigo