Molecular analysis of hantavirus isolated from Shandong province / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
; (6): 121-123, 2003.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-250526
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To compare the molecular characteristics of hantaviruses isolated from Shandong province by using PCR typing and nucleotide sequencing.</p><p><b>METHODS</b>Total cellular RNA was extracted from hantaviruses infected Vero E6 cells, viral cDNA was amplified by polymerase chain reaction. PCR products of partial M Segments of 4 strains of hantaviruses were sequenced. Cross neutralization tests were performed.</p><p><b>RESULTS</b>Four strains of hantavirus isolated from Rottus in Shandong province were SEO like viruses. The homology between Shandong isolates and other SEO like viruses was high at both amino acid and nucleotide levels. The homology among those 4 strains of hantaviruses was 98%.</p><p><b>CONCLUSIONS</b>The SEO like hantaviruses were more conserved than other hantaviruses. The homology of SEO like hantaviruses isolated from Shandong province was as high as 98% at nucleotides level, though they were isolated at more than 10-year intervals.</p>
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Filogenia
/
Variação Genética
/
Virologia
/
Reservatórios de Doenças
/
DNA Viral
/
Dados de Sequência Molecular
/
Sequência de Bases
/
Homologia de Sequência do Ácido Nucleico
/
China
/
Reação em Cadeia da Polimerase
Limite:
Animais
/
Humanos
País/Região como assunto:
Ásia
Idioma:
Chinês
Revista:
Chinese Journal of Experimental and Clinical Virology
Ano de publicação:
2003
Tipo de documento:
Artigo