Inhibition of Luciferase expression in mammalian cells by AAV vector plasmid mediated Luciferase shRNA / 中华实验和临床病毒学杂志

ABSTRACT

<p><b>OBJECTIVE</b>Constructing a plasmid containing the shRNA of luciferase to suppress the expression of luciferase in BHK-21 cell.</p><p><b>METHODS</b>A 334 bp human U6 snRNA promoter was amplified from human genomic DNA by PCR and ligated to a 21 bp reverse repeated motif of luciferase target sequence with 9 bp spacer and AAV plasmid pSNAV. The recombinant pSNAV/U6/Luc plasmid cotransfected with pMAMneoLuc or transfected luciferase cell line to detect the effect of luciferase expression separately.</p><p><b>RESULTS</b>pSNAV/U6/luc suppresses the luciferase expression from pMAMneoLuc by 50% and luciferase cell line by 70%.</p><p><b>CONCLUSIONS</b>The results showed that the short hairpin RNA of luciferase can efficiently suppress its expression in BHK-21.</p>