Evaluation by indirect immunofluorescent assay and enzyme linked immunosorbent assay of the dynamic changes of serum antibody responses against severe acute respiratory syndrome coronavirus / 中华医学杂志(英文版)
Chinese Medical Journal
; (24): 446-450, 2005.
Article
em En
| WPRIM
| ID: wpr-250908
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>BACKGROUND</b>Severe acute respiratory syndrome coronavirus (SARS-CoV) is a newly emerging virus that gives rise to SARS patients with high rates of infectivity and fatality. To study the humoral immune responses to SARS-CoV, the authors evaluated IgG and IgM specific antibodies in patients' sera.</p><p><b>METHODS</b>Two methods, enzyme-linked immunosorbent assay (ELISA) and indirect immunofluorescent assay (IFA), were used to detect specific serum IgG and IgM against SARS-CoV in 98 SARS patients and 250 controls consisting of patients with pneumonia, health-care professionals and healthy subjects. The serum antibody profiles were investigated at different times over one and a half years in 18 of the SARS patients.</p><p><b>RESULTS</b>The sensitivity and specificity of ELISA for detecting IgG against SARS-CoV were 100.0% and 97.2% and for IgM 89.8% and 97.6% respectively; the figures using IFA for IgG were 100.0% and 100.0% and for IgM 81.8% and 100.0% respectively. During the first seven days of the antibodies trace test, no IgG and IgM were detected, but on day 15, IgG response increased dramatically, reaching a peak on day 60, remaining high up to day 180 and decreasing gradually until day 540. On day 15, IgM was detected, rapidly reached a peak, then declined gradually until day 180 when IgM was undetectable.</p><p><b>CONCLUSION</b>The detection of antibodies against SARS virus is helpful in the clinical diagnosis of SARS.</p>
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Base de dados:
WPRIM
Assunto principal:
Sangue
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Imunoglobulina G
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Imunoglobulina M
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Ensaio de Imunoadsorção Enzimática
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Linfócitos T Citotóxicos
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Técnica Indireta de Fluorescência para Anticorpo
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Síndrome Respiratória Aguda Grave
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Coronavírus Relacionado à Síndrome Respiratória Aguda Grave
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Alergia e Imunologia
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Anticorpos Antivirais
Limite:
Adult
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Aged
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Female
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Humans
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Male
Idioma:
En
Revista:
Chinese Medical Journal
Ano de publicação:
2005
Tipo de documento:
Article