Binding interactions of pefloxacin mesylate with bovine lactoferrin and human serum albumin / 浙江大学学报(英文版)(B辑:生物医学和生物技术)
Journal of Zhejiang University. Science. B
; (12): 452-458, 2006.
Artigo
em Inglês
| WPRIM (Pacífico Ocidental)
| ID: wpr-251902
Biblioteca responsável:
WPRO
ABSTRACT
The binding of pefloxacin mesylate (PFLX) to bovine lactoferrin (BLf) and human serum albumin (HSA) in dilute aqueous solution was studied using fluorescence spectra and absorbance spectra. The binding constant K and the binding sites n were obtained by fluorescence quenching method. The binding distance r and energy-transfer efficiency E between pefloxacin mesylate and bovine lactoferrin as well as human serum albumin were also obtained according to the mechanism of Förster-type dipole-dipole nonradiative energy-transfer. The effects of pefloxacin mesylate on the conformations of bovine lactoferrin and human serum albumin were also analyzed using synchronous fluorescence spectroscopy.
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Ligação Proteica
/
Conformação Proteica
/
Espectrometria de Fluorescência
/
Espectrofotometria Ultravioleta
/
Sítios de Ligação
/
Albumina Sérica
/
Cinética
/
Pefloxacina
/
Química
Limite:
Animais
/
Humanos
Idioma:
Inglês
Revista:
Journal of Zhejiang University. Science. B
Ano de publicação:
2006
Tipo de documento:
Artigo