Expressions of sperm-specific genes in carnitine-cultured testis sperm of obstructive azoospermia patients / 中华男科学杂志
National Journal of Andrology
; (12): 504-509, 2010.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-252794
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of carnitine on human sperm motility and its potential role in the treatment of male infertility diseases.</p><p><b>METHODS</b>We obtained sperm by testis puncture from obstructive azoospermia patients and cultured them in vitro with normal culture solution (the control group) and the solution with L-carnitine at the concentration of 100 and 250 mmol/L, respectively. We observed the changes in sperm motility and morphology before and after the treatment, detected the expressions of the germ-specific genes, Vasa, Dazl, Acr, Prm1 and ATPase 6.0 by RT-PCR, and investigated the relationship between L-carnitine and the genes associated with sperm development and maturation.</p><p><b>RESULTS</b>After 24 -72 hours of treatment, the percentage of motile sperm was significantly higher in the 100 mmol/L L-carnitine group than in the control and 250 mmol/L L-carnitine groups (P < 0.01); the number of forward moving sperm was obviously increased and sperm morphology remained normal in the 100 mmol/L L-carnitine group. RT-PCR showed that L-carnitine increased the expressions of Acr, Prm1, Dazl and ATPase 6. 0 at the concentration of 100 mmol/L, and decreased the expressions of Dazl, Acr and Prm1 at 250 mmol/L.</p><p><b>CONCLUSION</b>L-carnitine at a proper concentration may improve the motility of incubated testicular sperm by upregulating the expressions of some sperm-specific genes, which helps sperm selection for intracytoplasmic sperm injection. However, a higher concentration of L-carnitine may reduce the expressions of these genes, probably due to its increased toxicity.</p>
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Contagem de Espermatozoides
/
Espermatozoides
/
Testículo
/
Carnitina
/
Expressão Gênica
/
Células Cultivadas
/
Reação em Cadeia da Polimerase Via Transcriptase Reversa
/
Azoospermia
/
Genética
Limite:
Humanos
/
Masculino
Idioma:
Chinês
Revista:
National Journal of Andrology
Ano de publicação:
2010
Tipo de documento:
Artigo