CMV pp65 gene modified dendritic cells activate autologous T cells / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 397-400, 2008.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-253311
Biblioteca responsável:
WPRO
ABSTRACT
Cytomegalovirus (CMV) infection is a dangerous complication in patients with chronic graft versus host disease (cGVHD). CMV-specific immunity depends on the activity of T cells. This study was aimed to investigate the effect of CMV pp65 gene modified dendritic cells (DCs) on activation of autologous T cells. Lentivirus system was utilized to introduce the CMV full-length pp65 gene into mouse DCs; CpG-DNA was used to induce mature DCs; flow cytometry and immunofluorescence were used to determine the expression of antigen and IFNgamma in T lymphocytes. The results showed that the DCs were infected with lentivirus at a multiplicity of infection (MOI) of 50 with optimal infectious efficiency of 30%-40%; mature DCs expressing pp65 gene could stimulate autologous naive T cells to express CD69 specifically; mature DCs expressing PP65 could stimulate autologous CD4+ or CD8+ T cells to produce IFNgamma. It is concluded that CMV pp65-modified and CpG-DNA-induced mature DCs can activate CMV-specific T lymphocytes in vitro.
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Fosfoproteínas
/
Células Dendríticas
/
DNA
/
Linfócitos T CD4-Positivos
/
Antígenos de Diferenciação de Linfócitos T
/
Antígenos CD
/
Proteínas da Matriz Viral
/
Interferon gama
/
Lentivirus
/
Linfócitos T CD8-Positivos
Limite:
Animais
/
Humanos
Idioma:
Chinês
Revista:
Journal of Experimental Hematology
Ano de publicação:
2008
Tipo de documento:
Artigo