Changes of expression and distribution of prohibitin in oxidative stressed cardiomyocyte and its biological significance / 中国应用生理学杂志

ABSTRACT

<p><b>AIM</b>To approach the expression of prohibitin in oxidative stressed cardiomyocytes.</p><p><b>METHODS</b>The oxidative stress model was established by treating neonatal cardiomyocytes with H2O2. Injury of cardiomyocytes were evaluated by detecting the LDH activity and MTT cell survival rate.The expression level of prohibitin was examined via the Western-blotting. The ability of mitochondrial oxidative phosphorylation was determined by measuring ATP synthesis via H+ -ATPase. Mitochondrial membrane potential was detected by flow cytometry using Rhl23.</p><p><b>RESULTS</b>LDH activity increased significantly after exposure to H202, while the cell survival rate decreased by 34.51%-65.5%. The contents of mitochondrial prohibitin in stress group was much higher than that in control group. At the same time, the ability of ATP synthesis decreased by 60% and mitochondrial transmembrane potential decreased too.</p><p><b>CONCLUSION</b>Express of prohibitin in oxidative stress cardiomyocytes was compensated increase. Prohibitin translocated to mitochondria after oxidative stress. Oxidative stress led to mitochondrial dysfunction.</p>