NR2B-pERK1/2-pElk-1 signaling contributes to the avoidance learning and memory of rats / 中国应用生理学杂志
Chinese Journal of Applied Physiology
; (6): 121-125, 2007.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-253469
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>AIM</b>To investigate whether NR2B-pERK1/2-pElk-1 signaling contributes to the Y-maze learning and memory of rat brain.</p><p><b>METHODS</b>45 adult male SD rats were divided into 4 groups (1) Ifenprodil peritoneal injection group (Ifenprodil ip, n = 14); (2) DMSO peritoneal injection group(DMSO ip, n = 15); (3) Ifenprodil cerebral ventricle injection group (Ifenprodil ic, n = 8); (4) DMSO cerebral ventricle injection group(DMSO ic, n = 8). Y-maze training and test were used as an learning and memory enhancing stimulus. Immunohistochemical and Western blotting methods were used for detecting pERK1/2 and pElk-1 expression intensity of different brain regions.</p><p><b>RESULTS</b>Compared with the DMSO ip group, the ifenprodil ip group showed no change on the Y-maze learning score (P > 0.05), but its Y-maze memory score tested 24 after learning decreased (P < 0.05). Ifenprodil peritoneal injection made brain pERK1/2 and pElk-1 expression decreased generally. In hippocampus, marginal division of striatum(MrD), amygdala,these changes were more significant (P < 0.05). Compared with the DMSO ic group, the reconsolidation of Y-maze memory tested 6 hours after ifenprodil injection was impaired in ifenprodil ic group (P < 0.05). The OD value of pERK1/2 and pElk-1 positive bands in ifenprodil ic group attenuated generally. The pElk-1 positive bands of caudate putamen and MrD almost disappeared in ifenprodil ic group.</p><p><b>CONCLUSION</b>NR2B is essential for the formation of long-term memory, reconsolidation of Y-maze memory. The deactivation of NR2B by ifenprodil will impair these courses. Meanwhile, the deactivation of NR2B attenuates pERK1/2 and pElk-1 expression of learning and memory related regions after Y-maze learning and memory reconsolidation test. In MrD and caudate putamen, the pElk-1 expression are completely blocked by ifenprodil after memory reconsolidation test.</p>
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Fisiologia
/
Piperidinas
/
Aprendizagem da Esquiva
/
Dimetil Sulfóxido
/
Ratos Sprague-Dawley
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Receptores de N-Metil-D-Aspartato
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Aprendizagem em Labirinto
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MAP Quinases Reguladas por Sinal Extracelular
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Proteínas Elk-1 do Domínio ets
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Applied Physiology
Ano de publicação:
2007
Tipo de documento:
Artigo