Influence of enterococci on human sperm membrane in vitro / 亚洲男科学杂志(英文版)
Asian Journal of Andrology
; (6): 77-81, 2007.
Artigo
em Inglês
| WPRIM (Pacífico Ocidental)
| ID: wpr-253799
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>AIM</b>To study the influence of enterococci on human sperm membrane in vitro.</p><p><b>METHODS</b>Ejaculated human sperm were artificially infected with beta-hemolytic or non-beta-hemolytic enterococci at the bacteria sperm ratio of 501 at 37 degrees . Sperm membrane integrity was examined after incubation for 1, 3 and 5 h by hypoosmotic swelling (HOS) test and electron microscopy.</p><p><b>RESULTS</b>Sperm infected with beta-hemolytic enterococci had lower HOS scores compared with non-beta-hemolytic strains or uninfected control (P < 0.01). The HOS test scores of sperm infected with beta-hemolytic enterococci increased in the presence of phosphatidylcholine, an inhibitor of hemolysin. Non-beta-hemolytic strains showed no significant difference in swelling rate, compared with the control group (P > 0.05). It was shown by electron microscopy that beta-hemolytic enterococci caused significant rupture of human sperm membrane.</p><p><b>CONCLUSION</b>Beta-hemolytic enterococci caused human sperm membrane injury, and might be mediated by the hemolysin of enterococci.</p>
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Fosfatidilcolinas
/
Fisiologia
/
Valores de Referência
/
Espermatozoides
/
Membrana Celular
/
Enterococcus
/
Ejaculação
/
Fezes
/
Microbiologia
Limite:
Humanos
/
Masculino
Idioma:
Inglês
Revista:
Asian Journal of Andrology
Ano de publicação:
2007
Tipo de documento:
Artigo