Study of the subcellular location of the human gene 6 transactivated by nonstructeral protein 5a of hepatitis C virus / 中华实验和临床病毒学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To found the subcellular location of the human gene 6 transactivated by nonstructural protein 5A of hepatitis C virus (NS5ATP6).</p><p><b>METHODS</b>Green fluorescent protein (GFP) expression vector pEGFP- NS5ATP6 was established. The pEGFP- NS5ATP6 was transfected into HepG2 cells, and analyze the subcellular location of the proteins expressed by NS5ATP6 through Green fluorescent microscopy after 24 hours.</p><p><b>RESULTS</b>The pEGFP- NS5ATP6 gene was successful cloned, NS5ATP6 can express protein in cells and subcellularly located in cell plasma.</p><p><b>CONCLUSION</b>NS5ATP6 can express protein, and the protein expressed by NS5ATP6 subcellularly located in cell plasma.</p>