Relationship between expression of STAT3 gene and proliferation of rat pulmonary arterial smooth muscle cells under hypoxia conditions / 中国应用生理学杂志
Chinese Journal of Applied Physiology
; (6): 75-79, 2006.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-254601
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>AIM</b>To explore the relationship between expression of signal transduction and activators of transcription 3 (STAT3) gene and proliferation of rat pulmonary arterial smooth muscle cells (PASMCs) under hypoxia conditions.</p><p><b>METHODS</b>After primarily cultured rat PASMCs was treated with AG490 and then exposed to hypoxia, the tyrosine-phosphorylated STAT3 protein were detected at 2 h, 6 h, 12 h, 16 h, 24 h of exposure to hypoxia by semi-quantitive RT-PCR (sqRT-PCR) and Western blot respectively. The expression of c-myc mRNA was analyzed by sqRT-PCR. 3H-TdR incorporation was used to detect the cell proliferation.</p><p><b>RESULTS</b>The level of tyrosine-phosphorylated STAT3 increased at 6 h and peaked at 12 h. The expression of c-myc mRNA increased after 2 h of hypoxia and reached maximal level at 4 h, then declined at 6 h and to the basal levels at 12 h. With the prolonging of hypoxia time, 3H-TdR incorporation in PASMC under hypoxia conditions was significantly higher. AG490 inhibited proliferation of PASMCs by preventing STAT3 tyrosine phosphorylation and the expression of c-myc under hypoxia conditions.</p><p><b>CONCLUSION</b>(1) The activation of STAT3 and c-myc gene might play an important role in the early stage of hypoxia-induced PASMCs proliferation. (2) STAT3 upregulated the expression of c-myc during the proliferation of PASMCs induced by hypoxia.</p>
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Artéria Pulmonar
/
Transdução de Sinais
/
Hipóxia Celular
/
Células Cultivadas
/
Ratos Wistar
/
Biologia Celular
/
Miócitos de Músculo Liso
/
Proliferação de Células
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Fator de Transcrição STAT3
/
Hipóxia
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Applied Physiology
Ano de publicação:
2006
Tipo de documento:
Artigo