Effects of connective tissue growth factor antisense oligonucleotides on the cultured human keloid fibroblasts in vitro / 中华整形外科杂志
Chinese Journal of Plastic Surgery
; (6): 454-456, 2004.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-255123
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To explore the effects of connective tissue growth factor (CTGF) on the pathogenesis of human keloid.</p><p><b>METHODS</b>CTGF antisense oligonucleotides (ASODN) conjugated with isothiocyanate fluorescence was encapsulated by liposome, and then added into the human keloid fibroblast (HKF) culturing media. The intracellular distribution of CTGF ASODN was observed with fluorescence microscopy in the fixed HKF. The proliferation of HKF was measured by MIT test. The apoptosis of HKF was measured with a flow cytometer. The collagen synthesis of HKF was measured by using H3-proline incorporation method.</p><p><b>RESULTS</b>The CTGF ASODN inhibited the proliferation and collagen synthesis of the HKF, compared with the control, but it increased the apoptosis after the transfection (P < 0.01).</p><p><b>CONCLUSION</b>CTGF ASODN may has anti-fibrotic effects on human keloid in vitro, and the CTGF may play an important role in promoting the fibrosis of human keloid.</p>
Texto completo:
Disponível
Contexto em Saúde:
ODS3 - Meta 3.4 Reduzir as mortes prematuras devido doenças não transmissíveis
Problema de saúde:
Outras Neoplasias Malignas
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Patologia
/
Transfecção
/
Diferenciação Celular
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Células Cultivadas
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Oligonucleotídeos Antissenso
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Apoptose
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Biologia Celular
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Fator de Crescimento do Tecido Conjuntivo
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Fibroblastos
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Genética
Limite:
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Plastic Surgery
Ano de publicação:
2004
Tipo de documento:
Artigo