Neuroprotective effects of paeonol in a cell model of Parkinson disease / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
; (6): 30-36, 2015.
Article
em Zh
| WPRIM
| ID: wpr-255238
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of paeonol on neuron cell model of Parkinson disease (PD).</p><p><b>METHODS</b>The cell model of Parkinson disease was induced by treatment of 1-Methyl-4-phenylpyridinium (MPP+) in PC12 cells, the PD model cells were treated with 1 μmol/L, 3 μmol/L or 9 μmol/L paeonol for 24h, respectively. Cell viability and LDH leakage were detected by MTT and lactate dehydrogenase (LDH) assay; the apoptosis of PC12 cells was assessed by Hoechst 33258 staining and flow cytometry; reactive oxygen species (ROS) production was detected by DCFH-DA method; and the ratio of Bax/Bcl-2 and activation of caspase-3 were determined by Western blotting.</p><p><b>RESULTS</b>MPP+ treatment significantly reduced cell viability, increased LDH leakage, enhanced the proportion of apoptotic cells and ROS production. In addition, MPP+ treatment dramatically increased the Bax/Bcl-2 ratio, and the activation of caspase-3. Compared to PD model group, paeonol treatment significantly enhanced cell viability, decreased LDH leakage, inhibited the proportion of apoptotic cells and ROS production, reduced the Bax/Bcl-2 ratio and the activated caspase-3 protein.</p><p><b>CONCLUSION</b>Paeonol can prevent PC12 cells from apoptosis induced by MPP+, and the mechanism may be associated with the down-regulation of ROS production, Bax/Bcl-2 ratio and Caspase-3 activation.</p>
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1
Base de dados:
WPRIM
Assunto principal:
Doença de Parkinson
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Acetofenonas
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Farmacologia
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Regulação para Baixo
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1-Metil-4-fenilpiridínio
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Sobrevivência Celular
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Células PC12
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Espécies Reativas de Oxigênio
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Apoptose
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Fármacos Neuroprotetores
Limite:
Animals
Idioma:
Zh
Revista:
Journal of Zhejiang University. Medical sciences
Ano de publicação:
2015
Tipo de documento:
Article