Construction and identification of genomic cDNA subclones of dengue 2 virus NGC strain / 南方医科大学学报
Journal of Southern Medical University
; (12): 469-471, 2006.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-255282
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To construct the cDNA subclones spanning the entire genome of dengue 2 virus NGC strain for further construction of full-length infectious viral cDNA clone.</p><p><b>METHODS</b>Two pairs of primers were designed according to the restriction endonuclease sites in the viral genome of dengue 2 virus NGC strain. After viral RNA extraction from the brain of infected new-born mice, two parts of full-length viral cDNA were amplified by long RT-PCR and cloned into the vector pCR-XL-TOPO. The partial sequence of the recombinant plasmid was determined.</p><p><b>RESULTS AND CONCLUSION</b>Sequence analysis and digestion with restriction enzymes demonstrated that the two cDNA subclones were specific for dengue 2 virus NGC strain, suggesting the successful construction of the two cDNA subclones of dengue 2 virus NGC strain.</p>
Texto completo:
Disponível
Contexto em Saúde:
Doenças Negligenciadas
Problema de saúde:
Dengue
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Recombinação Genética
/
Virologia
/
Encéfalo
/
DNA Viral
/
RNA Viral
/
Classificação
/
Genoma Viral
/
Clonagem Molecular
/
DNA Complementar
/
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Limite:
Animais
Idioma:
Chinês
Revista:
Journal of Southern Medical University
Ano de publicação:
2006
Tipo de documento:
Artigo