Effect of Kv1.3 and KCa3.1 potassium ion channels on the proliferation and migration of monocytes/macrophages / 生理学报
Acta Physiologica Sinica
; (6): 505-512, 2015.
Article
em Zh
| WPRIM
| ID: wpr-255919
Biblioteca responsável:
WPRO
ABSTRACT
This study was aimed to investigate the effects of blockade of Ca(2+) activated channel KCa3.1 and voltage-gated potassium channel Kv1.3 of the monocytes/macrophages on inflammatory monocyte chemotaxis. Chemotaxis assay was used to test the inflammatory Ly-6C(hi) monocyte chemotaxis caused by the monocytes/macrophages. The proliferation of monocytes/macrophages was detected by cell counting kit-8 (CCK8). Enzyme-linked immunosorbent assay (ELISA) was applied to detect the C-C motif ligand 7 (CCL7) in cultured media. The results showed that the recruitment of Ly-6C(hi) monocyte induced by monocytes/macrophages was suppressed by the potent Kv1.3 blocker Stichodactyla helianthus neurotoxin (ShK) or the specific KCa3.1 inhibitor TRAM-34. Meanwhile, the proliferation of monocytes/macrophages was significantly inhibited by ShK. The response of Ly-6C(hi) monocyte pretreated with ShK or TRAM-34 to CCL2 was declined. These results suggest that KCa3.1 and Kv1.3 may play an important role in monocytes/macrophages' proliferation and migration.
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Farmacologia
/
Fisiologia
/
Pirazóis
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Ensaio de Imunoadsorção Enzimática
/
Monócitos
/
Movimento Celular
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Estrutura Terciária de Proteína
/
Venenos de Cnidários
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Biologia Celular
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Proliferação de Células
Limite:
Humans
Idioma:
Zh
Revista:
Acta Physiologica Sinica
Ano de publicação:
2015
Tipo de documento:
Article