The Antisense Oligodeoxynucleotides of bcl-2 Oncogene Enhances the Sensitivity of K562 Leukemic Cells to As(2)O(3) / 中国实验血液学杂志

ABSTRACT

The biological reaction, changes of cellular DNA and bcl-2 protein content of K562 cells to bcl-2 ASODN or As(2)O(3) alone or bcl-2 ASODN associated with As(2)O(3) were investigated by cellular microculture, immunological tissue-chemistry method, and flow cytometry. The results showed that there is much more induce-apoptotic effect of As(2)O(3) on K562 cells while it combined with bcl-2 ASODN (ASODN 10.0 micro mol/L + As(2)O(3) 5.0 micro mol/L) than bcl-2 ASODN or As(2)O(3) alone (ASODN 10.0 micro mol/L or As(2)O(3) 5.0 micro mol/L), (P < 0.01), and so does inhibitory effect of bcl-2 protein expression by flow cytometry (P < 0.01). In conclusions, the bcl-2 ASODN can enhance the drug-sensetivity of K562 leukemic cells to As(2)O(3).