Intracellular Antibody Fragment Against Hepatitis B Virus X Protein Does Not Inhibit Viral Replication
Yonsei Medical Journal
; : 721-728, 2006.
Artigo
em Inglês
| WPRIM (Pacífico Ocidental)
| ID: wpr-25916
Biblioteca responsável:
WPRO
ABSTRACT
Replication of the hepatitis B virus is suppressed by deficiency of the X protein. Although several molecules that block cellular targets of X protein reduce the production of hepatitis B virus progeny, the effect of a specific inhibitor of X protein on viral replication has not been investigated. To block X protein specifically, we adopted an intracellular expression approach using H7 single chain variable fragment (H7scFv), an antibody fragment against X protein. We previously demonstrated that cytoplasmic expression of H7scFv inhibits X protein-induced tumorigenicity and transactivation. In this study, intracellular H7scFv expression inhibits reporter gene transactivation but not viral replication determined by endogenous hepatitis B virus polymerase activity assay and real-time PCR. Our findings imply that intracellular expression of antibody fragment against X protein may not be an alternative therapeutic modality for inhibition of hepatitis B virus replication.
Texto completo:
Disponível
Contexto em Saúde:
ODS3 - Saúde e Bem-Estar
Problema de saúde:
Meta 3.3: Acabar com as doenças tropicais negligenciadas e combater as doenças transmissíveis
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Replicação Viral
/
Região Variável de Imunoglobulina
/
Transativadores
/
Linhagem Celular
/
Vírus da Hepatite B
/
Antígenos E da Hepatite B
Idioma:
Inglês
Revista:
Yonsei Medical Journal
Ano de publicação:
2006
Tipo de documento:
Artigo