Using single primer PCR to amplify the orf25 gene fragment related to plant male-sterility in Salicornia europaea / 生物工程学报
Chinese Journal of Biotechnology
; (12): 120-123, 2003.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-259182
Biblioteca responsável:
WPRO
ABSTRACT
The gene orf25 encodes functional protein that may play an important role in plant fertility control in nature. To clone the orf25 from Salicornia europaea Xinjiang into a T-vector, a single designed primer was used to amplify 1.7kb cDNA fragment with RT-PCR. Sequence analysis reveals that the cloned fragment contains entire orf25 coding region with 98%, 95%, 92% and 88% identity to that of orf25 from Beta vulgaris, Nicotiana, wheat and maize mitochondrion, respectively. This analysis suggests that orf25 gene is highly conserved in terms of evolution in plant; and it also suggests that wild plant Salicornia europaea contain a male-sterility gene similar to crops that is of great importance in improvement of the breed of crop.
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Fisiologia
/
Proteínas de Plantas
/
Dados de Sequência Molecular
/
Sequência de Bases
/
Homologia de Sequência do Ácido Nucleico
/
Química
/
Primers do DNA
/
Chenopodiaceae
/
Reação em Cadeia da Polimerase Via Transcriptase Reversa
/
Infertilidade das Plantas
Idioma:
Chinês
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2003
Tipo de documento:
Artigo