miR-328 Inhibits K562 Cell Proliferation by Up-regulation of C/EBPα Expression / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 350-355, 2015.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-259586
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>Our research was aim to investigate the effect of microRNA-328 (miR-328) on proliferation of chronic myeloid leukemia(CML) cell K562 and the mediated effect of C/EBPα.</p><p><b>METHODS</b>The eukaryotic expression vectors of miR-328 targeting gene and suppressor gene (hsa-miR-328 and hsa-miR-328-inhibitor) were constructed, and transfected into K562 cells respectively. The mRNA expression levels of miR-328 and C/EBP α were detected by real-time fluorescence quantitative RT-PCR; C/EBP α protein expression was detected by Western blot; CCK-8 was used to estimate the cell viability.</p><p><b>RESULTS</b>The recombinant genes of hsa-miR-328 and hsa-miR-328-inhibitor were successfully constructed and transfected into K562 cells. Fluorescent cells were observed after 24 h, and the visible fluorescence cells were gradually increased after 48 h or 72 h, the miR-328 showed no effect on the mRNA expression of C/EBPα detected by RT-PCR. Meanwhile, miR-328 showed recovering effect on C/EBPα translation and inhibition of K562 cells proliferation.</p><p><b>CONCLUSION</b>miR-328 has been successfully constructed and transfected into K562 cells, miR-328 inhibits the proliferation of K562 cells by up-regulation of C/EBPα.</p>
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Transfecção
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Leucemia Mielogênica Crônica BCR-ABL Positiva
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Regulação para Cima
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Sobrevivência Celular
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Células K562
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Proteína alfa Estimuladora de Ligação a CCAAT
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MicroRNAs
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Proliferação de Células
Limite:
Humanos
Idioma:
Chinês
Revista:
Journal of Experimental Hematology
Ano de publicação:
2015
Tipo de documento:
Artigo