A comparative study on inducing non-homologous mesenchymal stem cells to differentiate into neural stem cells using non-homologous cerebrospinal fluid / 生物医学工程学杂志
Journal of Biomedical Engineering
; (6): 1290-1297, 2013.
Article
em Zh
| WPRIM
| ID: wpr-259722
Biblioteca responsável:
WPRO
ABSTRACT
In order to set up a base for stem cells to be widely used in clinical medicine, we tried to optimize, in this study, the technique that induces human mesenchymal stem cells (hMSCs) to differentiate into neural stem cells by using cerebrospinal fluid (CSF) from the different groups. After the induction, presence of neural stem cells was confirmed with microscope observation, flow cytometry analysis, immunohistochemistry and fluorescent immunohistochemistry. At the same time, we also compared and analysed the data of the number of stem cells when it totally met the requirements for clinical treatment and the days required. At last, we confirmed that hMSCs could be induced to differentiate into neural stem cells, and that the number of cells totally met the requirements for clinical treatment. But there were some differences both in the number of cells and the days required. Among the groups, the group that marrow mesenchymal stem cells from patients own induced by CSF from healthy volunteers used the shortest time and the quantity of the cells was significantly higher than those of the others.
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Imuno-Histoquímica
/
Diferenciação Celular
/
Líquido Cefalorraquidiano
/
Química
/
Meios de Cultura
/
Biologia Celular
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Células-Tronco Neurais
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Células-Tronco Mesenquimais
/
Citometria de Fluxo
Limite:
Humans
Idioma:
Zh
Revista:
Journal of Biomedical Engineering
Ano de publicação:
2013
Tipo de documento:
Article