Construction and eukaryotic expression of PVAX1-hPV58mE6E7fcGB composite gene vaccine / 生物医学工程学杂志
Journal of Biomedical Engineering
; (6): 1102-1107, 2013.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-259759
Biblioteca responsável:
WPRO
ABSTRACT
To construct and express a composite gene vaccine for human papillomavirus 58(HPV58)-associated cervical cancer, we inserted HPV58mE6E7 fusion gene into pCI-Fc-GPI eukaryotic expression vector, constructing a recombinant plasmid named pCI-sig-HPV58mE6E7-Fc-GPI. Then we further inserted fragment of sig-HPV58mE6E7Fc-GPI into the novel vaccine vector PVAX1-IRES-GM/B7, constructing PVAX1-HPV58mE6E7FcGB composite gene vaccine. PVAX1-HPV58mE6E7FcGB vaccine was successfully constructed and identified by restriction endonuclease and sequencing analysis. Eukaryotic expression of fusion antigen sig-HPV58mE6E7-Fc-GPI and molecular ad-juvant GM-CSF and B7. 1 were proved to be realized at the same time by flow cytometry and immunofluorescence. So PVAX1-HPV58mE6E7FcGB can be taken as a candidate of therapeutic vaccine for HPV58-associated tumors and their precancerous transformations.
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Proteínas Recombinantes
/
Neoplasias do Colo do Útero
/
Proteínas Oncogênicas Virais
/
Vacinas Anticâncer
/
Vacinas de DNA
/
Proteínas do Capsídeo
/
Alergia e Imunologia
/
Proteínas E7 de Papillomavirus
/
Vacinas contra Papillomavirus
/
Genética
Limite:
Feminino
/
Humanos
Idioma:
Chinês
Revista:
Journal of Biomedical Engineering
Ano de publicação:
2013
Tipo de documento:
Artigo