Preparation of in-house reference for diphtheria vaccine

ABSTRACT

The IVAC in-house reference for diphtheria vaccine has an average dry weight of 51.18 mg/ampoule, 2.85% moisture content (standard 3%). The reference vaccine kept at -20oC for 60 months still remains stable with 333.84 IU/ampoule. Kept at 37oC for 1, 2, 4, 6 and 8 weeks still remains stable. Potency of diphtheria component of DPT vaccine controlled by in-house reference vaccine and international standard vaccine gave the same results.