Experimental study on the effects of the nerve growth factor regulating calcitonin gene-related peptide in promoting the proliferation of MG-63 in vitro / 华西口腔医学杂志
West China Journal of Stomatology
; (6): 234-237, 2015.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-261098
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the nerve growth factor (NGF) regulating the expression of calcitonin gene-related peptide (CGRP) in promoting the proliferation of osteoblast-like cell (MG-63) and thus illustrate the mechanism of the NGF in wound healing.</p><p><b>METHODS</b>Different concentrations of NGF were used to stimulate MG-63. The expression of CGRP was detected by real-time quantitative polymerase chain reaction (RT-QPCR) and enzyme-linked immunosorbent assay after 1, 2, 3, and 4 days. The proliferation of MG-63 was detected by cell counting kit-8 (CCK-8). The expression of CGRP mRNA and the proliferation of MG-63 were then detected by RT-QPCR and CCK-8 after adding the NGF receptor blocker.</p><p><b>RESULTS</b>Compared with the blank control group, the expression of CGRP significantly increased by stimulating the NGF. The expression of CGRP was positively related to the concentration of NGF (P<0.05). Moreover, the expression of CGRP increased by prolonging the NGF stimulation time. The proliferation of MG-63 increased after stimulating the NGF (P<0.05). After adding the NGF receptor blocker, the expression of CGRP and the proliferation of MG-63 correspondingly decreased (P<0.05).</p><p><b>CONCLUSION</b>NGF can up-regulate the expression of CGRP and increase the proliferation of MG-63. Therefore, NGF plays a significant role in wound healing.</p>
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Calcitonina
/
Transdução de Sinais
/
Peptídeo Relacionado com Gene de Calcitonina
/
Regulação para Cima
/
Linhagem Celular
/
Ratos Sprague-Dawley
/
Fator de Crescimento Neural
/
Receptor de Fator de Crescimento Neural
/
Gânglios Espinais
/
Metabolismo
Limite:
Animais
/
Humanos
Idioma:
Chinês
Revista:
West China Journal of Stomatology
Ano de publicação:
2015
Tipo de documento:
Artigo