Effects of mineral trioxide aggregate on the proliferation and differentiation of human pulp cells from primary and permanent teeth / 华西口腔医学杂志
West China Journal of Stomatology
; (6): 75-79, 2015.
Article
em Zh
| WPRIM
| ID: wpr-261134
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>This study aims to investigate the effects of mineral trioxide aggregate (MTA) and calcium hydroxide on proliferation and differentiation of human pulp cells from primary and permanent teeth.</p><p><b>METHODS</b>Cell proliferation was detected by methyl thiazolyl tetrazolium (MTT) assay. The mRNA expression levels of dentinogenesis-related factors, including alkaline phosphatase (ALP) and dentin sialophosphoprotein (DSPP), and odontoclastogenesis-related factors, such as osteo- protegerin (OPG) and receptor activator of NF-κB ligand (RANKL), were determined by real time polymerase chain reac- tion (PCR).</p><p><b>RESULTS</b>Primary and permanent pulp cells treated with calcium hydroxide exhibited significantly lower proli- feration rates than the control cells (P<0.01). By contrast, the MTA-treated group showed significantly higher proliferation rates than the control group (P<0.01). Real time PCR results showed that calcium hydroxide-treated primary pulp cells exhi- bited significantly decreased ALP, DSPP, and OPG expression compared with the control group (P<0.01). Conversely, the MTA-treated group displayed significantly increased ALP, DSPP, and OPG expression (P<0.01). Calcium hydroxide-treated primary pulp cells also exhibited significantly upregulated RANKL expression (P < 0.01); by contrast, MTA-treated cells did not show any change in RANKL expression (P>0.05). Likewise, MTA-treated permanent pulp cells showed significantly upregulated ALP and DSPP expression (P < 0.01). However, the calcium hydroxide-treated group remained almost the same as the control group (P > 0.05). Neither MTA nor calcium hydroxide affected OPG and RANKL expression in per- manent pulp cells (P > 0.05).</p><p><b>CONCLUSION</b>MTA is more suitable as a pulp-capping agent, particularly in primary teeth, than calcium hydroxide.</p>
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Óxidos
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Fosfoproteínas
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Sialoglicoproteínas
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Hidróxido de Cálcio
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Diferenciação Celular
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Proteínas da Matriz Extracelular
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Silicatos
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Compostos de Cálcio
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Compostos de Alumínio
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Dentição Permanente
Limite:
Humans
Idioma:
Zh
Revista:
West China Journal of Stomatology
Ano de publicação:
2015
Tipo de documento:
Article