Expression of B7 costimulator in eight human malignant hematological cell lines and its significance / 中华血液学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To explore the expression of B7 co-stimulator in human malignant hematological cell lines and its significance.</p><p><b>METHODS</b>Amplified and purified plasmid DNA was transfected by liposome and detection of B7 gene expression by flow cytometry and RT-PCR.</p><p><b>RESULT</b>Eight human malignant hematological cell lines U937, K562, CEM, Daudi, GM, PEER, Jurkat, Raji express high level of HLA molecules, low or undetectable level of B7-1 gene, and high level of B7-2 gene. Expression level of B7-1 gene was enhanced after B7-1 transfection. T cell expressed high level of IL-2 mRNA after B7-1 transfection.</p><p><b>CONCLUSION</b>Multiple human malignant hematological cell lines express low or undetectable level of B7-1 gene, suggesting that B7-1 plays a critical role in tumor immunity.</p>