Study on gene expression changes of arsenic sulfide treated NB4 cells / 中华血液学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To compare the gene expression status of NB4 cells before and after arsenic sulfide treatment by cDNA microarray.</p><p><b>METHODS</b>Two cDNA probes were made from mRNA of untreated or arsenic sulfide treated NB4 cells. The cells were labelled with Cy3 or Cy5 fluorescence dyes individually, hybridized with cDNA microarray, and scanned for fluorescent intensity. The altered gene expression was screened through the analysis of difference in gene expression profile.</p><p><b>RESULTS</b>Thirty four genes related to apoptosis, cell cycle and others expressed different after the treatment of arsenic sulfide, 28/34 were up-regulated, 6/34 down-regulated.</p><p><b>CONCLUSION</b>ABC50, PNAS-2 and cyclin G(2) might take part in the process of NB4 cell apoptosis induced by arsenic sulfide.</p>