Expression of DNA methyltransferase in myeloma U266 cells and its significance / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 1429-1431, 2011.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-261854
Biblioteca responsável:
WPRO
ABSTRACT
In order to study the activity and gene expression of DNA methyltransferase (DNMT) on U266 myeloma cells and to analyze their significance, the activity of DNMT was detected by ELISA, and the expressions of DNMT1, DNMT3a and 3b were analyzed by RT-PCR. U266 cells were treated by phenylhexyl isothiocyanate (PHI), and the change of activity and gene expression of DNMT were determined. The results indicated that the activity and expression of DNMT in U266 myeloma cells were higher, compared with normal control. After being treated by different concentration of PHI, U266 cells were driven into apoptosis and the activity of DNMT decreased obviously and the mRNA level of DNMT declined. It is concluded that the activity and gene expression of DNMT on U266 myeloma cells are higher, and DNMT may be a new therapeutic target of multiple myeloma.
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
RNA Mensageiro
/
Metilação de DNA
/
Linhagem Celular Tumoral
/
DNA (Citosina-5-)-Metiltransferases
/
DNA (Citosina-5-)-Metiltransferase 1
/
Genética
/
Metabolismo
/
Mieloma Múltiplo
Limite:
Humanos
Idioma:
Chinês
Revista:
Journal of Experimental Hematology
Ano de publicação:
2011
Tipo de documento:
Artigo