Effects of resveratrol on proliferation and apoptosis of TNF-alpha induced rheumatoid arthritis fibroblast-like synoviocytes / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 1878-1882, 2010.
Article
em Zh
| WPRIM
| ID: wpr-262235
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To observe the effect of resveratrol (Res) on in vitro proliferation and apoptosis of TNF-alpha induced rheumatoid arthritis fibroblast-like synoviocytes (RA FLS), and further to investigate the PI3 K/Akt/BAD signal mechanism.</p><p><b>METHOD</b>The inhibition rate of RA FLS was examined by MTT assay. Cell cycle and the amount of apoptotic cells was measured by flow cytometry. PI3K/Akt/BAD signal transduction proteins expression was measured by western blot.</p><p><b>RESULT</b>The living cells measured by MTT dose and time-dependently reduced in Res groups. In Res groups, the fraction of living cells in the S-phase and G2/M-phase decreased respectively, while that in G1-phase increased, the difference was statistically significant compared with the TNF-alpha group (P < 0.05). Flow cytometry demonstrated that the apoptosis rate increased with increased Res concentration. Res inhibited TNF-alpha induced phosphorylation of Akt and BAD in RA FLS.</p><p><b>CONCLUSION</b>Res can inhibit RA FLS proliferation and induce apoptosis through inhibition of PI3K/Akt/BAD signalling pathway. Res may provide a new therapeutic approach in treatment of RA.</p>
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Farmacologia
/
Artrite Reumatoide
/
Estilbenos
/
Membrana Sinovial
/
Ciclo Celular
/
Células Cultivadas
/
Fator de Necrose Tumoral alfa
/
Apoptose
/
Biologia Celular
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Proliferação de Células
Limite:
Aged
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Female
/
Humans
/
Male
Idioma:
Zh
Revista:
China Journal of Chinese Materia Medica
Ano de publicação:
2010
Tipo de documento:
Article