Tyrosinase inhibitor from Angelica dahurica roots / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To isolate and identify the tyrosinase inhibitory constituent from Angelica dahurica.</p><p><b>METHOD</b>A tyrosinase inhibitory constituent was isolated by silica gel column chromatography combined with a bioassay-linked HPLC method.</p><p><b>RESULT</b>An extract of heat-processed A. dahurica roots showed more potent tyrosinase inhibitory activity than that of the crude drug. Tyrosinase inhibitory furanocoumarin, 8-hydroxy-5-methoxypsoralen,was isolated. It express stronger tyrosinase inhibition than the known tyrosinase inhibitor, kojic acid (IC50 = 0.0907 mmol x L(-1)) with IC50 value of 0.0086 mmol x L(-1). Therefore, it is contributive to the increased tyrosinase inhibitory activity of the heat-processed A. dahurica.</p><p><b>CONCLUSION</b>The active constituent was 8-hydroxy-5-methoxypsoralen from the heat-processed A. dahurica.</p>