Immunomodulatory function of orally administered thymosin alpha1 / 浙江大学学报(英文版)(B辑:生物医学和生物技术)
Journal of Zhejiang University. Science. B
; (12): 873-876, 2005.
Artigo
em Inglês
| WPRIM (Pacífico Ocidental)
| ID: wpr-263284
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the immunological function of a yeast expression system for thymosin alpha1 (Talpha1).</p><p><b>METHODS</b>A constructed Talpha1 yeast expression system was used to investigate the immunological function of orally administered Talpha1. Dried yeast containing three different concentration of Talpha1 was fed to normal Balb/c mice and other Balb/c mice whose immunities were inhibited in advance by cyclophosphamide. Synthesized Talpha1 peptide was used as positive control and dried yeast with empty plasmid was used as negative control. CD4(+) and CD8(+) levels were detected by flow cytometry assay. TNF-alpha, IFN-gamma, IL-2, IL-6 and IL-10 levels were detected by liquid chip.</p><p><b>RESULTS</b>In normal Balb/c mice or immune inhibition Balb/c mice, CD8(+) levels were significantly increased. Especially in immune inhibition Balb/c mice, CD8(+) levels in synthesized Talpha1 group (18.77%+/-4.72%), small dose group (13.48%+/-6.17%) and large dose group (22.74%+/-1.09%) were significantly higher than that in empty yeast control group (7.49%+/-2.14%).</p><p><b>CONCLUSION</b>Orally administered Talpha1 has its certain immunomodulatory function.</p>
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Saccharomyces cerevisiae
/
Timosina
/
Proteínas Recombinantes
/
Linfócitos T CD4-Positivos
/
Administração Oral
/
Citocinas
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Linfócitos T CD8-Positivos
/
Ciclofosfamida
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Tratamento Farmacológico
/
Alergia e Imunologia
Limite:
Animais
Idioma:
Inglês
Revista:
Journal of Zhejiang University. Science. B
Ano de publicação:
2005
Tipo de documento:
Artigo