The inhibitory effect of human endostatin gene on tumor growth of tongue squamous cell carcinoma / 中华口腔医学杂志
Chinese Journal of Stomatology
; (12): 273-276, 2004.
Article
em Zh
| WPRIM
| ID: wpr-263394
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the expression of transfected human endostatin (hES) gene in tongue squamous cell carcinoma (TSCC) and its inhibitory effects on the growth of tumor cells in vivo.</p><p><b>METHODS</b>Lipofectamine-mediated hES gene was transferred into Tca8113 cells, selected with Blasticidin S; The stable transfected cells were inoculated in BALB/c mice, and then the growth of xenografts was observed. The hES and vascular endothelial growth factor (VEGF) protein expression of xenografts was detected by S-P immuno-histochemical assay. We also detected the microvessel density (MVD) of xenografts with Weidern's method and apoptotic index of the tumor cells by flow cytometry (FCM).</p><p><b>RESULTS</b>The hES protein expression of xenografts in experimental group was significantly higher than that in control group (P < 0.01), while the expression of VEGF protein was on the other way round (P < 0.01). MVD counting of xenografts in experimental group was lower than that in control group (P < 0.01). The mean apoptotic level of the tumor cells in control group was also lower than in experimental group (P < 0.01). In addition, the inhibitory rate to growth of xenografts induced by hES transfection was 78.9%.</p><p><b>CONCLUSIONS</b>hES gene can be transferred into TSCC cells and then induce corresponding protein expression efficiently in xenograft model, resulting in significantly inhibitory effects on the xenografts in vivo.</p>
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Neoplasias da Língua
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Células Tumorais Cultivadas
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Carcinoma de Células Escamosas
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Transfecção
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Terapia Genética
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Endostatinas
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Genética
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Camundongos Endogâmicos BALB C
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Camundongos Nus
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Transplante de Neoplasias
Tipo de estudo:
Prognostic_studies
Limite:
Animals
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Female
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Humans
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Male
Idioma:
Zh
Revista:
Chinese Journal of Stomatology
Ano de publicação:
2004
Tipo de documento:
Article