HPLC determination of 6-gingerol in Rhizoma Zingiberis Recens / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To determine the contents of 6-Gingerol in Rhizoma Zingiberis Recens.</p><p><b>METHOD</b>HPLC method was used, with Alltech C18 column, acetonitrile-methol-water (43552) as mobile phase with a flow rate of 0.8 mL.min-1, detecting wavelength 280 nm, and column temperature 35 degrees C.</p><p><b>RESULT</b>Retained time of 6-gingerol was near 19 min, showing a good recovery (98.2%) and linear correlation (r = 0.9999). The contents of 6-gingerol were 1.35-2.87 mg.g-1, and the water contents were 70.4-85.5% mL.g-1 in Rhizoma Zingiberis Recens.</p><p><b>CONCLUSION</b>The method is appropriate for the determination of 6-gingerol in Rhizoma Zingiberis Recens. Gingerol can be used as a chemical marker of the quality control of Rhizoma Zingiberis Recens.</p>